IHC ANTIBODY CROSS REACTIVITY VERIFICATION

But, the advantages and disadvantages of using a monoclonal versus a polyclonal antibody should be assessed depending on the research needs. The key requirement is that the specific epitope of interest be exposed. Primary antibodies from different species must be used to prevent secondary antibody cross-reactivity and improve specificity.īoth monoclonal and polyclonal antibodies can work for immunofluorescent detection. Multiplexing allows for target co-localization studies can be carried out. Different fluorochrome-labeled antibodies can be combined in a multiplex ICC/IHC. More than one protein can be detected through multiplexing. Selection of primary antibody based on host.

IHC ANTIBODY CROSS REACTIVITY VERIFICATION

Target specificity verification ensures the antibody is recognizing the target protein of interest. Functional application validation provides information on whether the antibody works in the said application. Invitrogen antibodies undergo a two-part testing approach: functional application validation* and targeted specificity verification. Antibody specificity becomes more important when studying post-translational modifications or specific isoforms of the protein. Specificity of antibodies has been a growing and understandable concern. After ascertaining that the antibody does in fact for these applications, it can be followed up with either direct or indirect immunofluorescence detection methods. An antibody for IF should also work for ICC or IHC. Not all antibodies work for all applications.

Ihc antibody cross reactivity